The operation of buses, trolleybuses and battery buses on uneven roads causes dynamic and variable amplitude loading of these road vehicles. Such loading can lead to fatigue damage to critical nodes of the body or other vehicle components. Stress-time histories are most often random processes. Such loading processes can only be determined with sufficient accuracy by measurement. Fatigue analysis of these processes is fundamentally different from dynamic analysis. Dynamic analyses are performed in the frequency domain; fatigue analyses must be performed in the time domain to determine the so-called load spectra (histograms of load cycles). Fatigue damage can then be estimated using an appropriate fatigue damage accumulation hypothesis. The contribution presents the results of extensive measurements on several vehicles and in various operating conditions. The so-called design stress spectra for bodywork dimensioning and load spectra for laboratory tests of their critical structural nodes are derived.